Journal: Operative Dentistry

Article Title: Extended Resin Composite Restorations: Techniques and Procedures

doi: 10.2341/15-212-lit

Figure Lengend Snippet: Figure 5. Mini-flap: (a) Preoperative photo showing tooth #5 MOD amal- gam restoration with a deep mesial subgingival margin. Temporary resto- ration in occlusal surface is where endodontic access was prepared. (b) Radiograph of tooth #5 shows suc- cessful endodontic treatment. Mesial margin approximates osseous crest. (c) Facial-lingual mini-flap. Tissue retraction is limited to the keratinized tissue but provides excellent access to mesial restoration margin. (d) Op- erative field isolated with rubber dam. Note that despite location of deep mesial margin and execution of mini- flap the rubber dam provides com- plete isolation and accessibility of what once was the deep subgingival margin. (e) Amalgam build-up com- pleted; single suture placed in inter- dental papilla.

Article Snippet: The use of special matrices may facilitate the restoration of these complex situations, and special matrix bands are available with cervical extensions (eg, Tofflemire matrix band #2 [Produits Dentaire SA, Vevey, Switzerland] or Contact Matrix Subgingival matrices [Danville Materials, San Ramon, CA]).

Techniques: Isolation

Journal: Frontiers in Microbiology

Article Title: Development of an in vitro Assay, Based on the BioFilm Ring Test ® , for Rapid Profiling of Biofilm-Growing Bacteria

doi: 10.3389/fmicb.2016.01429

Figure Lengend Snippet: Biofilm formation on 96 well polystyrene plates for the Gram-negative bacteria after 5 h of incubation at 37°C: (A) P. aeruginosa (Pa), (B) K. pneumoniae (Kp), and (C) R. mannitolilytica isolated from the IFO hospital. Images were obtained after magnetization of the plates on the Block Test and scanning with the Plate Reader. The reference laboratory strains are indicated in bold. Negative controls with only BHI medium and magnetic microparticles are circled in red.

Article Snippet: Conversely, the cBRT, which is based on the physical ability of the microorganisms to generate a biofilm matrix, which traps magnetic beads (Chavant et al., ), provides a direct measurement of the genuine ability of different bacterial strains to produce biofilm.

Techniques: Bacteria, Incubation, Isolation, Blocking Assay

Journal: Frontiers in Microbiology

Article Title: Development of an in vitro Assay, Based on the BioFilm Ring Test ® , for Rapid Profiling of Biofilm-Growing Bacteria

doi: 10.3389/fmicb.2016.01429

Figure Lengend Snippet: Biofilm formation on 96 well polystyrene plates for the Gram-positive bacteria after 5 h of incubation at 37°C. (A) S. aureus (Sa), (B) S. epidermidis (Se), and (C) different Gram-positive bacteria isolated from the IFO hospital. Images were obtained after magnetization of the plates on the Block Test and scanning with the Plate Reader. The reference laboratory strains are indicated in bold. Negative controls with only BHI medium and magnetic microparticles are circled in red.

Article Snippet: Conversely, the cBRT, which is based on the physical ability of the microorganisms to generate a biofilm matrix, which traps magnetic beads (Chavant et al., ), provides a direct measurement of the genuine ability of different bacterial strains to produce biofilm.

Techniques: Bacteria, Incubation, Isolation, Blocking Assay